バイオベース燃料・化学品研究部門プロジェクト

代表的な大型プロジェクト

  1. NEDO カーボンリサイクル実現を加速するバイオ由来製品生産技術の開発「データベース空間からの新規酵素リソースの創出」（2020–2026年度）（研究担当者：近藤昭彦 教授, 蓮沼誠久 教授）
  2. JST ALCA「亜リン酸を用いたロバスト且つ封じ込めを可能とする微細藻類の培養技術開発」（2019–2022年度）（研究担当者：蓮沼誠久 教授）
  3. NEDO 植物等の生物を用いた高機能品生産技術の開発（研究開発項目[4] 微生物による高機能品生産技術開発）「希少アミノ酸エルゴチオネイン高生産スマートセルの開発」（2019-2020年度）（研究担当者：石井　純 准教授）
  4. 文部科学省 地域イノベーション・エコシステム形成プログラム「バイオ経済を加速する革新技術：ゲノム編集・合成技術の事業化」（2017-2021年度）（研究担当者：近藤昭彦 教授）
  5. JST 未来社会創造事業「光駆動ATP再生系によるVmax細胞の創製」（2017-2021年度）（研究担当者：石井　純 准教授）
  6. JST A-STEP「微細藻類からのカロテノイド色素の生産技術開発」（2016-2021年度）（研究担当者：蓮沼誠久 教授）
  7. NEDO 植物等の生物を用いた高機能品生産技術の開発「高生産性微生物創製に資する情報解析システムの開発」（2016-2020年度）（研究担当者：近藤昭彦 教授，蓮沼誠久 教授，石井 純 准教授，柘植謙爾 客員准教授，酒井香奈江 特命教授）
  8. 内閣府 ImPACT「動的代謝解析による海洋性緑藻の油脂生合成発動メカニズムの解明と油脂高生産技術開発への応用」（2016-2018年度）（研究担当者：蓮沼誠久 教授）
  9. JST ALCA「転写と時計の改変によるラン藻炭素源供給の量的緩和とコハク酸生産」（2013-2018年度）（研究担当者：蓮沼誠久 教授）
  10. 文部科学省 バイオプロダクション次世代農工連携拠点（2008-2018年度）（研究担当者：近藤昭彦 教授，蓮沼誠久 教授，吉田健一 教授，竹中慎治 教授，今石浩正 教授，石井 純 准教授，田中 勉 准教授，川口秀夫 特命准教授，酒井香奈江 特命助教）

原核スマートセル研究グループ

  1. Sato, N., Kishida, M., Nakano, M., Hirata, Y., Tanaka T*. (2020) Metabolic engineering of shikimic acid-producing Corynebacterium glutamicum from glucose and cellobiose retaining its phosphotransferase system function and pyruvate kinase activities. Frontiers in Bioengineering and Biotechnology, 8, 569406
  2. Kawaguchi, H., Sazuka, T., Kondo, A. (2020) Complete and draft genome sequences of amino acid-producing Corynebacterium glutamicum strains ATCC 21799 and ATCC 31831 and their genomic islands. Microbiology Resource Announcements, 9(32), e00430-20
  3. Murai, K., Sasaki, D., Kobayashi, S., Yamaguchi, A., Uchikura, H., Shirai, T., Sasaki, K., Kondo, A., Tsuge, Y. (2020) Optimal ratio of carbon flux between glycolysis and the pentose phosphate pathway for amino acid accumulation in Corynebacterium glutamicum. ACS Synthetic Biology, 9(7), 1615-1622
  4. Natutaki, S., Sugawara, Y., Akada, K., Lee, I., Kondo, A. (2020) Case study on the environmental monitoring for biological manufacturing using Time-lapse Shadow Image Analysis. Biologicals, 66, 1-8
  5. Satowa, D., Fujiwara, R., Uchio, S., Nakano, M., Otomo, C., Hirata, T., Matsumoto, T., Noda, S., Tanaka, T., Kondo, A. (2020) Metabolic engineering of E. coli for improving mevalonate production to promote NADPH regeneration and enhance acetyl-CoA supply. Biotechnology and Bioengineeering, 117(7), 2153-2164
  6. Wijaya H., Sasaki, K., Kahar, P., Rahmani, N., Hermiati, E., Yopi, Ogino, C., Prasetya, B., Kondo, A. (2020) High enzymatic recovery and purification of xylooligosaccharides from empty fruit bunch via nanofiltration. Processes, 8, 619
  7. Kobayashi, S., Kawaguchi, H., Shirai, T., Ninomiya, K., Takahashi, K., Kondo, A., Tsuge, Y. (2020) Automatic redirection of carbon flux between glycolysis and pentose phosphate pathway using an oxygen-responsive metabolic switch in Corynebacterium glutamicum. ACS Synthetic Biology, 9, 814-826
  8. Fujiwara, R., Noda, S., Tanaka, T., Kondo, A. (2020) Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose–xylose co-substrate. Nature Communications, 11: 279
  9. Matsumoto, T., Mori, Y., Tanaka, T., Kondo, A. (2020) n-Butylamine production from glucose using a transaminase-mediated synthetic pathway in Escherichia coli. Journal of Bioscience and Bioengineering, 129(1), 99-103
  10. Kobayashi, J., Kondo, A. (2019) Complete genome sequence of Rhodobacter sphaeroides HJ strain, a purple nonsulfur bacterium with high hydrogen production ability from acetate. Microbiology Resource Announcements, 8(37), e00652-19
  11. Noda, S., Mori, Y., Oyama, S., Kondo, A., Araki, M., Shirai, T. (2019) Reconstruction of metabolic pathway for isobutanol production in Escherichia coli. Microbial Cell Factories, 18: 124
  12. Matsuura, R., Kishida, M., Konishi, R., Hirata, Y., Adachi, N., Segawa, S., Imao, K., Tanaka, T., Kondo, A. (2019) Metabolic engineering to improve 1,5-diaminopentane production from cellobiose using β-glucosidase-secreting Corynebacterium glutamicum. Biotechnology and Bioengineering, 116(10), 2640-2651
  13. Kawaguchi, H., Miyagawa, H., Nakamura-Tsuruta, S., Takaya, N., Ogino, C., Kondo, A. (2019) Enhanced phenyllactic acid production in Escherichia coli via oxygen limitation and shikimate pathway gene expression. Biotechnology Journal, 14(6): 1800478
  14. Vavricka, CJ., Yoshida, T., Kuriya, Y., Takahashi, S., Ogawa, T., Ono, F., Agari, K., Kiyota, H., Li, J., Ishii, J., Tsuge, K., Minami, H., Araki, M., Hasunuma, T., Kondo, A. (2019) Mechanism-based tuning of insect 3,4-dihydroxyphenylacetaldehyde synthase for synthetic bioproduction of benzylisoquinoline alkaloids. Nature Communications, 10, 2015
  15. Takenaka, M., Lee, JM., Kahar, P., Ogino, C., Kond, A. (2019) Efficient and supplementary enzyme cocktail from Actinobacteria and plant biomass induction. Biotechnology Journal, 1700744
  16. Kobayashi, J., Kondo, A. (2019) Disruption of poly-3-hydroxyalkanoates depolymerase gene and overexpression of three poly-3-hydroxybutyrate biosynthetic genes improve poly-3-hydroxybutyrate production from nitrogen rich medium by Rhodobacter sphaeroides. Microbial Cell Factories, 18: 40
  17. Rahmani, N., Kahar, P., Lisdiyanti, P., Lee, J., Yopi., Prasetya, B., Ogino, C., Kondo, A. (2019) GH-10 and GH-11 Endo-1,4-β-xylanase enzymes from Kitasatospora sp. produce xylose and xylooligosaccharides from sugarcane bagasse with no xylose inhibition. Bioresource Technology, 272, 315-325
  18. Okano, K., Honda, K., Taniguchi, H., Kondo, A. (2018) De novo design of biosynthetic pathways for bacterial production of bulk chemicals and biofuels. FEMS Microbiology Letters, 365(20), fny215
  19. Fujiwara, R., Noda, S., Tanaka, T., Kondo, A. (2018) Muconic acid production using gene-level fusion proteins in Escherichia coli. ACS Synthetic Biology, 7(11), 2698-2705
  20. Noguchi, Y.#, Kashiwagi, N.#, Uzura, A., Ogino, C., Kondo, A., Ikeda, H., Sota, M. (2018) Development of a strictly regulated xylose-induced expression system in Streptomyces. Microbial Cell Factories, 17: 151
  21. Restu, WK., Nishida, Y., Yamamoto, S., Ishii, J., Maruyama, T. (2018) Short oligopeptides for biocompatible and biodegradable supramolecular hydrogels. Langmuir, 34, 8065-8074
  22. Tsuge, Y., Kawaguchi, H., Yamamoto, S., Nishigami, Y., Sota, M., Ogino, C., Kondo, A. (2018) Metabolic engineering of Corynebacterium glutamicum for production of sunscreen shinorine, Bioscience. Biotechnology, and Biochemistry, 82(7): 1252-1259
  23. Sasaki, K., Sasaki, D., Tsuge, Y., Morita, M., Kondo, A. (2018) Changes in the microbial consortium during dark hydrogen fermentation in a bioelectrochemical system increases methane production during a two-stage process. Biotechnology for Biofuels, 11: 173
  24. Tominaga, D., Kawaguchi, H., Hori, Y., Hasunuma, T., Ogino, C., Aburatani, S. (2018) Mathematical model for small size time series data of bacterial secondary metabolic pathways, Bioinformatics and Biology Insights, 12: 1-7
  25. Noguchi, Y., Kashiwagi, N., Uzura, A., Ogino, C., Kondo, A., Ikeda, H., Sota, M. (2018) Development of a strictly regulated xylose-induced expression system in Streptomyces, Microbial Cell Factories, 17: 151
  26. Kawaguchi, H., Yoshihara, K., Hara, KY., Hasunuma, T., Ogino, C., Kondo, A. (2018) Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of D-glucose and L-arabinose, Microbial Cell Factories, 17: 76
  27. Tashiro, Y., Hirano, S., Matson, MM., Atsumi, S., Kondo, A. (2018) Electrical-biological hybrid system for CO2 reduction. Metabolic Engineering, 47, 211-218
  28. Thomson, NM., Shirai, T., Chiapello, M., Kondo, A., Mukherjee, KJ., Sivaniah, E., Numata, K., Summers, DK. (2018) Efficient 3-hydroxybutyrate production by quiescent Escherichia coli microbial cell factories is facilitated by indole-induced proteomic and metabolomic changes. Biotechnology Journal, 13(5), 1700571
  29. Okano, K., Uematsu, G., Hama, S., Tanaka, T., Noda, H., Kondo, A., Honda, K. (2018) Metabolic engineering of Lactobacillus plantarum for direct L-lactic acid production from raw corn starch. Biotechnology Journal, 13(5), 1700517
  30. Gonçalves, G.A.L., Mori, Y., Tanaka, T., Kamiya, N. (2018) Casein-based scaffold for artificial cellulosome design. Process Biochemistry, 66, 140-145
  31. Rahmani, N., Kahar, P., Lisdiyanti, P., Hermiati, Euis, Lee, J., Yopi, Y., Prasetya, B., Ogino, C., Kondo, A. (2018) Xylanase and feruloyl esterase from actinomycetes cultures could enhance sugarcane bagasse hydrolysis in the production of fermentable sugars. Bioscience, Biotechnology, and Biochemistry, 82(5), 904-915
  32. Matsumoto, T., Isogawa, Y., Tanaka, T., Kondo, A. (2018) Streptavidin-hydrogel prepared by sortase A-assisted click chemistry for enzyme immobilization on an electrode. Biosensors and Bioelectronics, 99, 56-61
  33. Miyano, M., Tanaka, K., Ishikawa, S., Takenaka, S., Miguel-Arribas, A., Meijer, WJJ., Yoshida, KI. (2018) Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer. Microbial Cell Factories 17(1):13

真核スマートセル研究グループ

  1. Ito, Y., Terai, G., Ishigami, M., Hashiba, N., Nakamura, Y., Bamba, T., Kumokita, R., Hasunuma, T., Asai, K., Ishii, J.*, Kondo, A.* (2020) Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression. Nucleic Acids Research, in press
  2. Nag, A., Ali, MA., Kawaguchi, H., Saito, S., Kawasaki, Y., Miyazaki, S., Kawamoto, H., Nugroho Adi, DT., Yoshihara, K., Masuo, S., Katsuyama, Y., Kondo, A., Ogino, C., Takaya, N., Kaneko, T.*, Ohnishi, Y. (2020) Ultrahigh thermoresistant lightweight bioplastics developed from fermentation products of cellulosic feedstock. Advanced Sustainable Systems, 202000193
  3. Yukawa, T., Bamba, T., Guirimand, G., Matsuda, M., Hasunuma, T.*, Kondo, A*. Optimization of 1,2,4-butanetriol production from xylose in Saccharomyces cerevisiae by metabolic engineering of NADH/NADPH balance. Biotechnology and Bioengineering, in press
  4. Guirimand, G., Kulagina, N., Papon, N., Hasunuma, T.*, Courdavault, V.* (2020) Innovative tools and strategies for optimizing yeast cell factories. Trends in Biotechnology, in press
  5. Cunha, JT., Romani, A., Inokuma, K., Johansson, B., Hasunuma, T., Kondo, A., Domingues, L. (2020) Consolidated bioprocessing of corn cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts. Biotechnology for Biofuel, 13(1), 1-15
  6. Quayson, E., Amoah, J., Rachmadona, N., Morita, K., Darkwah, L., Hama, S., Yoshida, A., Kondo, A., Ogino, C. (2020) Valorization of palm biomass waste into carbon matrices for the immobilization of recombinant Fusarium heterosporum lipase towards palm biodiesel synthesis. Biomass and Bioenergy, 142, 105768
  7. Zhang, S., Wakai, S., Sasakura, N., Tsutsumi, H., Hata, Y., Ogino, C., Kondo, A. (2020) Pyruvate metabolism redirection for biological production of commodity chemicals in aerobic fungus Aspergillus oryzae. Metabolic Engineering, 61, 225-237
  8. Wijaya, H., Sasaki, K., Kahar, P., Quayson, E., Rachmadona, N., Amoah, J., Hama, S., Ogino, C., Kondo, A. (2020) Concentration of lipase from Aspergillus oryzae expressing Fusarium heterosporum by nanofiltration to enhance transesterification. Processes, 8(4), 450
  9. Quayson, E., Amoah, J., Rachmadona, N., Hama, S., Yoshida, A., Kondo, A., Ogino, C. (2020) Biodiesel-mediated biodiesel production: A recombinant Fusarium heterosporum lipase-catalyzed transesterification of crude plant oils. Fuel Processing Technology, 199, 106278
  10. Watanabe, N., Murata, M., Ogawa, T., Vavricka, CJ., Kondo, A., Ogino, C., Araki, M. (2020) Exploration and evaluation of machine learning-based models for predicting enzymatic reactions. Journal of Chemical Information and Modeling. 60(3), 1833-1843
  11. Inokuma, K., Kurono, H., den Haan, R., van Zyl, W. H., Hasunuma, T., Kondo, A. (2020) Novel strategy for anchorage position control of GPI-attached proteins in the yeast cell wall using different GPI-anchoring domains. Metabolic Engineering, 57, 110-117
  12. Bamba, T., Yukawa, T., Guirimand, G., Inokuma, K., Sasaki, K., Hasunuma, T., Kondo, A. (2019) Production of 1,2,4-butanetriol from xylose by Saccharomyces cerevisiae through Fe metabolic engineering. Metabolic Engineering, 56: 17-27
  13. Huang, X., Bai, S., Liu, Z., Hasunuma, T., Kondo, A., Ho, SH. (2019) Fermentation of pigment-extracted microalgal residue using yeast cell-surface display: direct high-density ethanol production with competitive life cycle impacts. Green Chemistry, 22(1), 153-162
  14. Morita, K., Matsuda, F., Okamoto, K., Ishii, J., Kondo, A., Shimizu, H. (2019) Repression of mitochondrial metabolism for cytosolic pyruvate-derived chemical production in Saccharomyces cerevisiae. Microbial Cell Factories, 18, 177
  15. Amoah, J., Ogura, K., Schmetz, Q., Kondo, A., Ogino, C. (2019) Co-fermentation of xylose and glucose from ionic liquid pretreated sugar cane bagasse for bioethanol production using engineered xylose assimilating yeast. Biomass & Bioenergy, 128
  16. Amza, RL., Kahar, P., Juanssifero, AB., Miyamoto, N., Otsuka, H., Kihira, C., Ogino, C., Kondo, A. (2019) High cell density cultivation of Lipomyces starkeyi for achieving highly efficient lipid production from sugar under low C/N ratio. Biochemical Engineering Journal, 149, 107236
  17. Guirimand, GGY., Bamba, T., Matsuda, M., Inokuma, K., Morita, K., Kitada, Y., Kobayashi, Y., Yukawa, T., Sasaki, K., Ogino, C., Hasunuma, T., Kondo, A. (2019) Combined cell surface display of β-D-glucosidase (BGL), maltose transporter (MAL11) and overexpression of cytosolic xylose reductase (XR) in Saccharomyces cerevisiae enhance cellobiose/xylose coutilization for xylitol bio-production from lignocellulosic biomass. Biotechnology Journal, 14(9): e1800704
  18. Juanssilfero, AB., Kahar, P., Amza, RL., Yopi, Sudesh, K., Ogino, C., Prasetya, B., Kondo, A. (2019) Lipid production by Lipomyces starkeyi using sap squeezed from felled old oil palm trunks. Journal of Bioscience and Bioengineering, 127(6), 726-73
  19. Schmetz, Q., Teramura, H., Morita, K., Oshima, T., Richel, A., Ogino, C., Kondo, A. (2019) Versatility of a dilute acid/butanol pretreatment investigated on various lignocellulosic biomasses to produce lignin, monosaccharides and cellulose in distinct phases. ACS Sustainable Chemistry & Engineering, 7(13), 11069-11079
  20. Guirimand, G., Inokuma, K., Bamba, T., Matsuda, M., Morita, K., Sasaki, K., Ogino, C., Berrin, JG., Hasunuma, T., Kondo, A. (2019) Cell-surface display technology and metabolic engineering of Saccharomyces cerevisiae for enhancing xylitol production from woody biomass. Green Chemistry, 21, 1795-1808
  21. Tamano, K., Cox Ⅲ, RS., Tsuge, K., Miura, A., Itoh, A., Ishii, J., Tamura, T., Kondo, A., Machida, M. (2019) Heterologous production of free dihomo-ɤ-linolenic acid by Aspergillus oryzae and its extracellular release via surfactant supplementation. Journal of Bioscience and Bioengineering, 127, 451-457
  22. Quayson, E., Jerome, A., Hama, S., Yoshida, A., Morita, K., Kondo, A., Ogino, C. (2019) Valorization of activated carbon as a reusable matrix for the immobilization of Aspergillus oryzae whole-cells expressing Fusarium heterosporum lipase toward biodiesel synthesis. ACS Sustainable Chemistry & Engineering, 7(5), 5010-5017
  23. Sakihama, Y., Hidese, R., Hasunuma, T., Kondo, A. (2019) Increased flux in acetyl-CoA synthetic pathway and TCA cycle of Kluyveromyces marxianus under respiratory conditions. Scientific Reports, 9(1): 5319
  24. Wakai, S., Nakashima, N., Ogino, C., Tsutsumi, H., Hata, Y., Kondo, A. (2019) Modified expression of multi-cellulases in a filamentous fungus Aspergillus oryzae. Bioresource Technology, 276: 146-153
  25. Kobayashi, J., Sasaki, D., Bamba, T., Hasunuma, T., Kondo, A. (2019) Sustainable production of glutathione from lignocellulose-derived sugars using engineered Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 103(3), 1243-1254
  26. Itoh, H., Matsui, M., Miyamura, Y., Takeda, I., Ishii, J., Kumagai, T., Machida, M., Shibata, T., Arita, M. (2018) Biosynthesis of Novel Statins by Combining Heterologous Genes from Xylaria and Aspergillus. ACS Synthetic Biology, 7(12), 2783-2789
  27. Ito, Y., Watanabe, T., Aikawa, S., Nishi, T., Nishiyama, T., Nakamura, Y., Hasunuma, T., Okubo, Y., Ishii, J., Kondo, A. (2018) Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii, FEMS Yeast Research, 18(7)
  28. Takayama, S., Ozaki, A., Konishi, R., Otomo, C., Kishida, M., Hirata, Y., Matsumoto, T., Tanaka, T., Kondo, A. (2018) Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe. Microbial Cell Factories, 17, 176
  29. Zhang, Y., Nakagawa, K., Shibuya, M., Sasaki, K., Takahashi, T., Shintani, T., Yoshida, T., Kamio, E., Kondo, A., Matsuyama, H. (2018) Improved permselectivity of forward osmosis membranes for efficient concentration of pretreated rice straw and bioethanol production. Journal of Membrane Science, 566, 15-24
  30. Wijaya, H., Sasaki, K., Kahar, P., Yopi, Kawaguchi, H., Sazuka, T., Ogino, C., Prasetya, B., Kondo, A. (2018) Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain. Bioresource Technology, 265, 542-547
  31. Inokuma, K., Matsuda M., Sasaki, D., Hasunuma, T., Kondo, A. (2018) Widespread effect of N-acetyl-D-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis. Microbial Cell Factories, 17, 153
  32. Juanssilfero, BA., Kahar, P., Amza, LR., Miyamoto, N., Otsuka, H., Matsumoto, H., Kihira, C., Thontowi, A., Yopi., Ogino, C., Prasetya, B., Kondo, A. (2018) Selection of oleaginous yeasts capable of high lipid accumulation during challenges from inhibitory chemical compounds. Biochemical Engineering Journal, 137, 182-191
  33. Hama, S., Kihara, M., Noda, H., Kondo, A. (2018) Development of cell recycle technology incorporating nutrient supplementation for lignocellulosic ethanol fermentation using industrial yeast Saccharomyces cerevisiae. Biochemical Engineering Journal, 137, 23-29
  34. Eguchi, Y., Makanae, K., Hasunuma, T., Ishibashi, Y., Kito, K., Moriya, H. (2018) Estimating the protein burden limit of yeast cells by measuring expression limits of glycolytic proteins. eLife, 7: e34595
  35. Nakamura, Y., Nishi, T., Noguchi, R., Ito, Y., Watanabe, T., Nishiyama, T., Aikawa, S., Hasunuma, T., Ishii, J., Okubo, Y., Kondo, A. (2018) A stable, autonomously replicating plasmid vector containing Pichia pastoris centromeric DNA, Applied and Environmental Microbiology, 84(15), e02882-17
  36. Ohno, R., Teramura, H., Ogino, C., Kondo, A., Takumi, S. (2018) Genotypic effects on sugar and by-products of liquid hydrolysates and on saccharification of acid-insoluble residues of from wheat straw. Genes & Genetic Systems, 93, 1-7
  37. Kobayashi, J., Sasaki, D., Kondo, A. (2018) A procedure for precise determination of glutathione produced by Saccharomyces cerevisiae. Bio-protocol, 8(12): e2887
  38. Juanssilfero, AB., Kahar, P., Amza, RL., Miyamoto, N., Otsuka, H., Matsumoto, H., Kihira, C., Thontowi, A., Yop, Ogino, C., Prasetya, B., Kondo, A. (2018) Effect of inoculum size on single-cell oil production from glucose and xylose using oleaginous yeast Lipomyces starkeyi. Journal of Bioscience and Bioengineering, 126(6), 695-702
  39. Inokuma, K., Matsuda, M., Sasaki, D., Hasunuma, T., Kondo, A. (2018) Widespread effect of N-acetyl-D-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis, Microbial Cell Factories, 17, 153
  40. Ishii, J., Morita, K., Ida, K., Kato, H., Kinoshita, S., Hataya, S., Shimizu, H., Kondo, A., Matsuda, F. (2018) A pyruvate carbon flux tugging strategy for increasing 2,3-butanediol production and reducing ethanol subgeneration in the yeast Saccharomyces cerevisiae, Biotechnology for Biofuels, 11: 180
  41. Teramura, H., Sasaki, K., Oshima, T., Kawaguchi, H., Ogino, C., Sazuka, T., Kondo, A. (2018) Effective usage of sorghum bagasse: optimization of organosolv pretreatment using 25% 1-butanol and subsequent nanofiltration membrane separation, Bioresource Technology, 252, 157-164
  42. Bamba, T., Inokuma, K., Hasunuma, T., Kondo, A. (2018) Enhanced cell-surface display of a heterologous protein using SED1 anchoring system in SED1-disrupted Saccharomyces cerevisiae strain. Journal of Bioscience and Bioengineering, 125(3), 306-310
  43. Nishimura, Y., Matsui, T., Ishii, J., Kondo, A. (2018) Metabolic engineering of the 2-ketobutyrate biosynthetic pathway for 1-propanol production in Saccharomyces cerevisiae, Microbial Cell Factories, 17: 38
  44. Li, YH., Zhang, XY., Zhang, F., Peng, LC., Zhang, DB., Kondo, A., Bai, FW., Zhao, X. (2018) Optimization of cellulolytic enzyme components through engineering Trichoderma reesei and on-site fermentation using the soluble inducer for cellulosic ethanol production from corn stover. Biotechnology for Biofuels, 11: 49
  45. Nambu-Nishida, Y., Sakihama, Y., Ishii, J., Hasunuma, T., Kondo, A. (2018) Selection of yeast Saccharomyces cerevisiae promoters available for xylose cultivation and fermentation. Journal of Bioscience and Bioengineering, 125(1), 76-86

CO2直接変換研究グループ

  1. Iijima, H., Watanabe, A., Sukigara, H., Shirai, T., Kondo, A., Osanai, T. (2020) Simultaneous increases in the levels of compatible solutes by cost‐effective cultivation of Synechocystis sp. PCC 6803. Biotechnology and Bioengineering, 117(6), 1649-1660
  2. Qu, W., Show, PL., Hasunuma, T., Ho, SH. (2020) Optimizing real swine wastewater treatment efficiency and carbohydrate productivity of newly microalga Chlamydomonas sp. QWY37 used for cell-displayed bioethanol production. Bioresource Technology, 305, 123072
  3. Hidese, R.#, Matsuda, M.#, Osanai, T., Hasunuma, T.*, Kondo, A. (2020) Malic enzyme facilitates D-lactate production through increased pyruvate supply during anoxic dark fermentation in Synechocystis sp. PCC 6803. ACS Synthetic Biology, 9, 260-268
  4. Aikawa, S., Nishida, A., Hasunuma, T.*, Chang, JS. Kondo, A. (2019) Short-term temporal metabolic behavior in halophilic cyanobacterium Synechococcus sp. strain PCC 7002 after salt shock. Metabolites, 9(12), 297
  5. Hasunuma, T.*, Takaki, A., Matsuda, M., Kato, Y., Vavricka, CJ., Kondo, A. (2019) Single-stage astaxanthin production enhances the nonmevalonate pathway and photosynthetic central metabolism in Synechococcus sp. PCC 7002. ACS Synthetic Biology, 8(12), 2701-2709
  6. Chen, JH., Kato, Y., Matsuda, M., Chen, CY., Nagarajan, D., Hasunuma, T., Kondo, A., Dong, CD., Lee, DJ., Chang, JS. (2019) A novel process for the mixotrophic production of lutein with Chlorella sorokiniana MB-1-M12 using aquaculture wastewater. Bioresource Technology, 290, 121786
  7. Miyagishima, S., Era, A., Hasunuma, T., Matsuda, M., Hirooka, S., Sumiya, N., Kondo, A. Fujiwara, T. (2019) Day/night separation of oxygenic energy metabolism and nuclear DNA replication in the unicellular red alga Cyanidioschyzon merolae. mBio, 10(4): e00833-19
  8. Chen, JH., Chen, CY., Hasunuma, T., Kondo, A., Chang, CH. Ng, IS., Chang, JS. (2019) Enhancing lutein production with mixotrophic cultivation of Chlorella sorokiniana MB-1-M12 using different bioprocess operation strategies. Bioresource Technology, 278, 17-25
  9. Ueno, Y., Aikawa, S., Kondo, A., Akimoto, S. (2019) Adaptation of light-harvesting functions of unicellular green algae to different light qualities. Photosynthesis Research, 139(1-3), 145-154
  10. Kato, Y., Fujihara, Y., Vavricka, CJ., Chang, JS., Hasunuma, T., Kondo, A. (2019) Light/dark cycling causes delayed lipid accumulation and increased photoperiod-based biomass yield by altering metabolic flux in oleaginous Chlamydomonas sp. Biotechnology for Biofuels, 12: 39
  11. J. Amoah, Hasunuma, T., Ogino, C., Kondo, A. (2019) 5-Hydroxymethylfurfural production from salt-induced photoautotrophically cultivated Chlorella sorokiniana. Biochemical Engineering Journal, 142, 117-123
  12. Hasunuma, T., Matsuda, M., Kato, Y., Vavricka, CJ., Kondo, A. (2018) Temperature enhanced succinate production concurrent with increased central metabolism turnover in the cyanobacterium Synechocystis sp. PCC 6803, Metabolic Engineering, 48, 109-120
  13. Aikawa, S., Inokuma, K., Wakai, S., Sasaki, K., Ogino, C., Chang, JS., Hasunuma, T., Kondo, A. (2018) Direct and highly productive conversion of cyanobacteria Arthrospira platensis to ethanol with CaCl2 addition, Biotechnology for Biofuels, 11: 50

総説

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