バイオベース燃料・化学品研究部門プロジェクト

代表的な大型プロジェクト

  1. 文部科学省 バイオプロダクション次世代農工連携拠点(2008-2018年度)(研究担当者:近藤昭彦 教授)
  2. 文部科学省 地域イノベーション・エコシステム形成プログラム「バイオ経済を加速する革新技術:ゲノム編集・合成技術の事業化」(2017-2021年度) (研究担当者:近藤昭彦 教授,西田敬二 教授,柘植謙爾 特命准教授,佐々木建吾 特命准教授)
  3. AMED 次世代治療・診断実現のための創薬基盤技術開発事業「国際基準に適合した次世代抗体医薬等の製造技術のうち高生産宿主構築の効率化基盤技術開発に係るもの」(2014-2017年度)(研究担当者:近藤昭彦 教授,石井 純 准教授,柘植謙爾 特命准教授,伊藤洋一郎 特命助教,中村泰之 特命助教)
  4. AMED 次世代治療・診断実現のための創薬基盤技術開発事業 バイオ医薬品の高度製造技術の開発「高性能な国産細胞株の構築」(2018-2020年度)(研究担当者:近藤昭彦 教授,石井 純 准教授,柘植謙爾 特命准教授,伊藤洋一郎 特命助教,中村泰之 特命助教,西村勇哉 特命助教,佐々木大介 特命助教)
  5. AMED 次世代治療・診断実現のための創薬基盤技術開発事業 バイオ医薬品の高度製造技術の開発「バイオ医薬品の多品種・大量 製造に適した微生物による高度生産技術の開発」(2018-2020年度)(研究担当者:石井 純 准教授,佐々木大介 特命助教)
  6. NEDO 植物等の生物を用いた高機能品生産技術の開発「高生産性微生物創製に資する情報解析システムの開発」(2016-2020年度)(研究担当者:近藤昭彦 教授,蓮沼誠久 教授,石井 純 准教授,柘植謙爾 特命准教授,酒井香奈江 特命教授)
  7. NEDO 植物等の生物を用いた高機能品生産技術の開発(研究開発項目[4] 微生物による高機能品生産技術開発)「希少アミノ酸エル ゴチオネイン高生産スマートセルの開発」(2019-2020年度) (研究担当者:石井 純 准教授,蓮沼誠久 教授)
  8. JST ALCA「転写と時計の改変によるラン藻炭素源供給の量的緩和とコハク酸生産」(2013-2018年度) (研究担当者:蓮沼誠久 教授)
  9. JST A-STEP「微細藻類からのカロテノイド色素の生産技術開発」(2016-2021年度)(研究担当者:蓮沼誠久 教授)
  10. JST 未来社会創造事業「光駆動ATP再生系によるVmax細胞の創製」(2017ー2021年度)(研究担当者:石井 純 准教授)
  11. 内閣府 ImPACT「動的代謝解析による海洋性緑藻の油脂生合成発動メカニズムの解明と油脂高生産技術開発への応用」(2016-2018年度)(研究担当者:蓮沼誠久 教授)

原核スマートセル研究グループ

  1. Tominaga, D., Kawaguchi, H., Hori, Y., Hasunuma, T., Ogino, C., Aburatani, S. (2018) Mathematical model for small size time series data of bacterial secondary metabolic pathways, Bioinformatics and Biology Insights, 12: 1-7
  2. Noguchi, Y., Kashiwagi, N., Uzura, A., Ogino, C., Kondo, A., Ikeda, H., Sota, M. (2018) Development of a strictly regulated xylose-induced expression system in Streptomyces, Microbial Cell Factories, 17: 151
  3. Kawaguchi, H., Yoshihara, K., Hara, KY., Hasunuma, T., Ogino, C., Kondo, A. (2018) Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of D-glucose and L-arabinose, Microbial Cell Factories, 17: 76
  4. Miyano, M., Tanaka, K., Ishikawa, S., Takenaka, S., Miguel-Arribas, A., Meijer, WJJ., Yoshida, KI. (2018) Rapid conjugative mobilization of a 100 kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer. Microbial Cell Factories 17(1):13
  5. Tanaka, K., Natsume, A., Ishikawa, S., Takenaka, S., Yoshida, KI. (2017) A new-generation of Bacillus subtilis cell factory for further elevated scyllo-inositol production. Microbial Cell Factories 16:67.
  6. Kono, T., Mehrotra, S., Endo, C., Kizu, N., Matsuda, M., Kimura, H., Mizohata, E., Inoue, T., Hasunuma, T., Yokota, A., Matsumura, H., Ashida, H. (2017) A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea, Nature Communications, 8 : 14007
  7. Kawaguchi, H., Katsuyama, Y., Danyao, D., Kahar, P., Nakamura-Tsuruta, S., Teramura, H., Wakai, K., Yoshihara, K., Minami, H., Ogino, C., Ohnishi, Y., Kondo, A. (2017) Caffeic acid production by simultaneous saccharification and fermentation of kraft pulp using recombinant Escherichia coli, Applied Microbiology and Biotechnology, 101(13), 5279–5290
  8. Kawaguchi, H., Sasaki, K., Uematsu, K., Tsuge, Y., Teramura, H., Okai, N., Nakamura-Tsuruta, S., Katsuyama, Y., Sugai, Y., Ohnishi, Y., Hirano, K., Sazuka, T., Ogino, C., Kondo, A. (2015) 3-Amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant Corynebacterium glutamicum, Bioresource Technology, 198: 410-417
  9. Kawaguchi, H., Teramura, H., Uematsu, K., Hara, KY., Hasunuma, T., Hirano, K., Sazuka, T., Kitano, H., Tsuge, Y., Kahar, P., Niimi-Nakamura, S., Oinuma, KI., Takaya, N., Kasuga, S., Ogino, C., Kondo, A. (2015) Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse, Bioresource Technology, 182:169-178
  10. Tsuge, K., Sato, Y., Kobayashi, Y., Gondo, M., Hasebe, M., Togashi, T., Tomita, M., Itaya, M. (2015) Method of preparing an equimolar DNA mixture for one-step DNA assembly of over 50 fragments, Scientific Reports, 5, 10655

真核スマートセル研究グループ

  1. Ito, Y., Watanabe, T., Aikawa, S., Nishi, T., Nishiyama, T., Nakamura, Y., Hasunuma, T., Okubo, Y., Ishii, J., Kondo, A. (2018) Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii, FEMS Yeast Research, 18(7)
  2. Nakamura, Y., Nishi, T., Noguchi, R., Ito, Y., Watanabe, T., Nishiyama, T., Aikawa, S., Hasunuma, T., Ishii, J., Okubo, Y., Kondo, A. (2018) A stable, autonomously replicating plasmid vector containing Pichia pastoris centromeric DNA, Applied and Environmental Microbiology, 84(15), e02882-17
  3. Inokuma, K., Matsuda, M., Sasaki, D., Hasunuma, T., Kondo, A. (2018) Widespread effect of N-acetyl-D-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis, Microbial Cell Factories, 17, 153
  4. Nishimura, Y., Matsui, T., Ishii, J., Kondo, A. (2018) Metabolic engineering of the 2-ketobutyrate biosynthetic pathway for 1-propanol production in Saccharomyces cerevisiae, Microbial Cell Factories, 17: 38
  5. Nambu-Nishida, Y., Nishida, K., Hasunuma, T., Kondo, A. (2017) Development of a comprehensive set of tools for genome engineering in a cold- and thermo-tolerant Kluyveromyces marxianus yeast strain, Scientific Reports, 7(1): 8993
  6. Liu, Z., Inokuma, K., Ho, SH., den Haan, R., van Zyl, WH., Hasunuma, T., Kondo, A. (2017) Improvement of ethanol production from crystalline cellulose via optimizing cellulase ratios in cellulolytic Saccharomyces cerevisiae. Biotechnology and Bioengineering, 114(6), 1201-1207
  7. Kobayashi, J., Sasaki, D., Hara, KY., Hasunuma, T., Kondo, A. (2017) Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae, Microbial Cell Factories, 16:44
  8. Ishii, J., Morita, K., Ida, K., Kato, H., Kinoshita, S., Hataya, S., Shimizu, H., Kondo, A., Matsuda, F. (2018) A pyruvate carbon flux tugging strategy for increasing 2,3-butanediol production and reducing ethanol subgeneration in the yeast Saccharomyces cerevisiae, Biotechnology for Biofuels, 11: 180
  9. Yamamoto, K., Hara, KY., Morita, T., Nishimura, A., Sasaki, D., Ishii, J., Ogino, C., Kizaki, N., Kondo, A. (2016) Enhancement of astaxanthin production in Xanthophyllomyces dendrorhous by efficient method for the complete deletion of genes, Microbial Cell Factories,15(1):155
  10. Guirimand, G., Sasaki, K., Inokuma, K., Bamba, T., Hasunuma, T., Kondo, A. (2016) Cell-surface engineering of Saccharomyces cerevisiae combined with membrane separation technology for xylitol production from rice straw hydrolysate, Applied Microbiology and Biotechnology, 100(8), 3477-3487
  11. Ida, K., Ishii, J., Matsuda, F., Kondo, T., Kondo, A. (2015) Eliminating the isoleucine biosynthetic pathway to reduce competitive carbon outflow during isobutanol production by Saccharomyces cerevisiae, Microbial Cell Factories ,14: 62
  12. Wakai, S., Yoshie, T., Asai-Nakashima, N., Yamada, R., Ogino, C., Tsutsumi, H., Hara, KY., Kondo, A. (2014) L-lactic acid production from starch by simultaneous saccharification and fermentation in a genetically engineered Aspergillus oryzae pure culture, Bioresource Technology, 173, 376-383
  13. Yamada, R., Yoshie, T., Wakai, S., Asai-Nakashima, N., Okazaki, F., Ogino, C., Hisada, H., Tsutsumi, H., Hhata, Y., Kondo, A. (2014) Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose, Microbial Cell Factories, 13:71

CO2直接変換研究グループ

  1. Hasunuma, T., Matsuda, M., Kato, Y., Vavricka, CJ., Kondo, A. (2018) Temperature enhanced succinate production concurrent with increased central metabolism turnover in the cyanobacterium Synechocystis sp. PCC 6803, Metabolic Engineering, 48, 109-120
  2. Aikawa, S., Inokuma, K., Wakai, S., Sasaki, K., Ogino, C., Chang, JS., Hasunuma, T., Kondo, A. (2018) Direct and highly productive conversion of cyanobacteria Arthrospira platensis to ethanol with CaCl2 addition, Biotechnology for Biofuels, 11: 50
  3. Amoah, J., Ho, SH., Hama, S., Yoshida, A., Nakanishi, A., Hasunuma, T., Ogino, C., Kondo, A. (2017) Conversion of Chlamydomonas sp. JSC4 lipids to biodiesel using Fusarium heterosporum lipase-expressing Aspergillus oryzae whole-cell as biocatalyst, Algal Research, 28, 16-23
  4. Ho, SH., Nakanishi, A., Kato, Y., Yamasaki, H., Chang, JS., Misawa, N., Hirose, Y., Minagawa, J., Hasunuma, T., Kondo, A. (2017) Dynamic metabolic profiling together with transcription analysis reveals salinity-induced starch-to-lipid biosynthesis in alga Chlamydomonas sp. JSC4, Scientific Reports, 4;7: 45471
  5. Wang, Y., Chiu, SY., Ho, SH., Liu, Z., Hasunuma, T., Chang, TT., Chang, KF., Chang, JS., Ren, NQ., Kondo, A. (2016) Improving carbohydrate production of Chlorella sorokiniana NIES-2168 through semi-continuous process coupled with mixotrophic cultivation, Biotechnology Journal,11(8), 1072-1081
  6. Aikawa, S., Nishida, A., Ho, SH., Chang, JS., Hasunuma, T., Kondo, A. (2014) Glycogen production for biofuels by the euryhaline cyanobacteria Synechococcus sp. strain PCC 7002 from an oceanic environment, Biotechnology for Biofuels, 7:88
  7. Hasunuma, T., Miyazawa, S., Yoshimura, S., Shinzaki, Y., Tomizawa, K., Shindo, K., Choi, SK., Misawa, N., Miyake, C. (2008) Biosynthesis of astaxanthin in tobacco leaves by transplastomic engineering, Plant Journal, 55(5), 857-868.

総説

  1. 蓮沼誠久, 田村具博, 近藤昭彦, 総論, スマートセルインダストリー -微生物細胞を用いた物質生産の展望-, 1-9, シーエムシー出版 (2018)
  2. Kawaguchi, H., Ogino, C., Kondo, A. (2017) Microbial conversion of biomass into bio-based polymers, Bioresource Technology, 245(Part B), 1664-1673
  3. Wakai, S., Arazoe, T., Ogino, C., Kondo, A. (2017) Future insights in fungal metabolic engineering. Bioresource Technology, 245, 1314-1326
  4. Kashiwagi, N., Ogino, C., Kondo, A. (2017) Production of chemicals and proteins using biomass-derived substrates from a Streptomyces host, Bioresource Technology, 245, 1655-1663
  5. Kawaguchi, H., Hasunuma, T., Ogino, C., Kondo, A. (2016) Bioprocessing of bio-based chemicals produced from lignocellulosic feedstocks., Current Opinion in Biotechnology, 42: 30-39
  6. Hasunuma, T., Ishii, J., Kondo, A. (2015) Rational design and evolutional fine tuning of Saccharomyces cerevisiae for biomass breakdown., Current Opinion in Chemical Biology, 29: 1-9
  7. Aikawa, S., Ho, SH., Nakanishi, A., Chang, JS., Hasunuma, T., Kondo, A. (2015) Improving polyglucan production in cyanobacteria and microalgae via cultivation design and metabolic engineering., Biotechnology Journal, 10(6), 886-898
  8. Tsuge, Y., Hasunuma, T., Kondo, A. (2015) Recent advances in the metabolic engineering of Corynebacterium glutamicum for the production of lactate and succinate from renewable resources., Journal of Industrial Microbiology & Biotechnology, 42(3), 375-389
  9. Hara, KY., Araki, M., Okai, N., Wakai, S., Hasunuma, T., Kondo, A. (2014) Development of bio-based fine chemical production through synthetic bioengineering, Microbial Cell Factories, 13(1): 173
  10. Ho, SH., Ye, X., Hasunuma, T., Chang, JS., Kondo, A. (2014) Perspectives on engineering strategies for improving biofuel production from microalgae - A critical review., Biotechnology Advances, 32, 1448-1459
  11. Hasunuma, T., Okazaki, F., Okai, N., Hara, KY., Ishii, J., Kondo, A. (2013) A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology, Bioresourse Technology , 135, 513-522
  12. Kondo, A., Ishii, J., Hara, KY., Hasunuma, T., Matsuda, F. (2013) Development of microbial cell factories for bio-refinery through synthetic bioengineering., Journal of Biotechnololgy, 163(2), 204-216
  13. Hasunuma, T., Kondo, A. (2012) Consolidated bioprocessing and simultaneous saccharification and fermentation of lignocellulose to ethanol with thermotolerant yeast strains., Process Biochemistry, 47(9), 1287-1294
  14. Hasunuma, T., Kondo, A., Miyake, C. (2009) Metabolic pathway engineering by plastid transformation is a powerful tool for production of compounds in higher plants., Plant Biotechnology, 26(1), 39-46