機能性食品素材・アグロバイオ研究部門プロジェクト

代表的な大型プロジェクト

  1. 文部科学省 バイオプロダクション次世代農工連携拠点（2008-2018年度）（研究担当者：芦田 均教授，大澤 朗教授，金丸研吾准教授，水野雅史教授，橋本堂史准教授，山崎将紀准教授，本田和久准教授）

アグロバイオ研究グループ

  1. Komatsu, A., Ohtake, M., Shimatani, Z., Nishida, K. (2020) Production of herbicide-sensitive strain to prevent volunteer rice infestation using a CRISPR-Cas9 cytidine deaminase fusion., Front Plant Sci., 11: 925 https://doi.org/10.3389/fpls.2020.00925
  2. Seki, K., Komatsu, K., Tanaka, K., Hiraga, M., Kajiya-Kanegae, H., Matsumura, H., Uno, Y. (2020) A CIN-like TCP transcription factor (LsTCP4) having retrotransposon insertion associates with a shift from Salinas type to Empire type in crisphead lettuce (Lactuca sativa L.). Hort Res., 7:15(14 pages) https://doi.org/10.1038/s41438-020-0241-4
  3. Fujikura, U., Ezaki, K., Horiguchi, G., Seo, M., Kanno, Y., Kamiya, Y., Lenhard, M., Tsukaya H. (2020) Suppression of class I compensated cell enlargement by xs2 mutation is mediated by salicylic acid signaling. PLoS Genet., 25;16(6):e1008873.
  4. Furuhata, Y., Sakai, A., Murakami, T., Morikawa, M., Nakamura, C., Yoshizumi, T., Fujikura, U., Nishida, K., Kato, Y. (2019) A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall. Sci Rep., 15;9(1):2163.
  5. Ohno, R., Teramura, H., Ogino, C., Kondo, A., Takumi, S. (2018) Genotypic effects on sugar and by-products of liquid hydrolysates and on saccharification of acid-insoluble residues from wheat straw. Genes Genet Syst., 93(1):1-7 https://doi.org/10.1266/ggs.17-00027
  6. Shimatani, Z., Fujikura, U., Ishii, H., Matsui, Y., Suzuki, M., Ueke, Y., Taoka, K., Terada, R., Nishida, K., Kondo,A. (2018) Inheritance of co-edited genes by CRISPR-based targeted nucleotide substitutions in rice., Plant Physiol Biochem., 131: 78-83 https://doi.org/10.1016/j.plaphy.2018.04.028
  7. Shimatani, Z., Fujikura, U., Ishii, H., Terada, R., Nishida, K., Kondo, A. (2018) Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice, Data in Brief, 20: 1325-1331 https://doi.org/10.1016/j.dib.2018.08.124
  8. Ishibashi, M., Nabe, T., Nitta, Y., Tsuruta, H., Iduhara, M., Uno, Y. (2018) Analysis of major paralogs encoding the Fra a 1 allergen based on their organ-specificity in Fragaria × ananassa. Plant Cell Report, 37(3):411–424 https://doi.org/10.1007/s00299-017-2237-6
  9. Omidbakhshfard, MA., Fujikura, U., Olas, JJ., Xue, GP., Balazadeh, S., Mueller-Roeber, B. (2018) GROWTH-REGULATING FACTOR 9 negatively regulates arabidopsis leaf growth by controlling ORG3 and restricting cell proliferation in leaf primordia. PLoS Genet 9;14(7):e1007484

機能性食品素材腸内動態研究グループ

  1. Yoshida, N., Yamashita, T., Kishino, S., Watanabe, H., Sasaki, K., Sasaki, D., Tabata, T., Sugiyama, Y., Kitamura, N., Saito, Y., Emoto, T., Hayashi, T. Takahashi, T., Shinohara, M., Osawa, R., Kondo, A., Yamada, T., Ogawa, J., Hirata, K. (2020) A possible beneficial effect of Bacteroides on faecal lipopolysaccharide activity and cardiovascular diseases. Scientific Reports, 10, Article number: 13009.
  2. Hayashi D., Wang L., Ueda S., Yamanoue M., Ashida H., Shirai Y. (2020) The mechanisms of ameliorating effect of a green tea polyphenol on diabetic nephropathy based on diacylglycerol kinase α. Sci Rep., 10:11790-11802
  3. Mizuno, M., Sakaguchi, K., Sakane, I. (2020) Oral administration of fucoidan can exert anti-allergic activity after allergen sensitization by enhancement of galectin-9 secretion in blood. Biomolecules, 10, 258, https://doi.org/10.3390/biom10020258
  4. Oka, S., Ohto, N., Kuwahara, H., Mizuno, M. Oral (2020) administration of pineapple glucosylceramide improves defective epidermal barrier function by restoring diminished level of TGF-β in the skin, Eur. Food Res. Technol., 246, 867-874.
  5. Fujii, M., Fukuura, K., Ohto, N., Kuwahara, H., Mizuno, M. (2020) Lactobacillus plantarum 22A-3 exerts anti-allergic activity through TGF-β secretion in passive cutaneous anaphylaxis of mice, Int. J. Food Sci. Nutr., https://doi.org/10.1080/09637486.2020.1833316.
  6. Yoshida, N., Sasaki, K., Sasaki, D., Yamashita, T., Fukuda, H., Hayashi, T., Tabata, T., Osawa, R., Hirata, K., Kondo, A. (2019) Impact of resistant starch on the gut microbiota and its metabolites in a single-batch fermentation system varies among fecal samples from patients with coronary artery disease. Journal of Atherosclerosis and Thrombosis, 26 :705-719.
  7. Shinohara, R., Sasaki, K., Inoue, J., Hoshi, N., Fukuda, I., Sasaki, D., Kondo, A., Osawa, R. (2019) Butyryl-CoA:acetate CoA-transferase gene associated with the genus Roseburia is decreased in the gut microbiota of Japanese patients with ulcerative colitis. Bioscience of Microbiota, Food and Health, 38: 159-163.
  8. Minato, K., Laan, L.C., van Die, I., Mizuno, M. (2019) Pleurotus citrinopileatus polysaccharide stimulates anti-inflammatory properties during monocyte-to-macrophage differentiation, Int. J. Biol. Macromol., 122, 705-712.
  9. Sasaki, D., Sasaki, K., Ikuta, N., Yasuda, T., Fukuda, I., Kondo, A., Osawa, R. (2018) Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure. Scientific Reports, 8, Article number: 435
  10. Sakaguchi, K., Shirai, Y., Itoh, T. Mizuno, M. (2018) Lentinan exerts its anti-inflammatory activity by suppressing TNFR1 transfer to the surface of intestinal epithelial cells through Dectin-1 in an in vitro and mice model, Immunome Res., 14, 165. DOI: 10.4172/1745-7580.1000165.
  11. Hayashi, D., Ueda, S., Yamanoue, M., Ashida, H,. Shirai, Y. (2018) Amelioration of diabetic nephropathy by oral administration of d-α-tocopherol and its mechanisms. Biosci Biotechnol Biochem., 82, 65-73.

機能性食品素材体内動態研究グループ

  1. Yamashita, Y., Wang, L., Nakamuea, A., Nanba, F., Saito, S., Toda, T., Nakagawa, J., Ashida, H. (2020) Black soybean improves the vascular function through an increase in nitric oxide and a decrease in oxidative stress in healthy women. Arch Biochem Biophys, 688, 1080408.
  2. Yamashita, Y., Nakamuea, A., Nanba, F., Saito, S., Toda, T., Nakagawa, J., Ashida, H. (2020) Black soybean improves vascular function and blood pressure: A Randomized, placebo controlled, crossover trial in humans. Nutrients, 12, 2755.
  3. Wang, L., Yamashita, Y., Komeda, S., Saito, A., Ashida,H. Absorption, metabolism, distribution and faecal excretion of B-type procyanidin oligomers in mice after a single oral administration of black soybean seed coat extract. Food Funct, 9, 5362-5370 (2018)

総説

  1. Yamashita, Y., Sakakibara, H., Toda, T., Ashida, H. (2020) Insights into the potential benefits of black soybean (Glycine max L.) polyphenols in lifestyle diseases. Food Funct, 11, 7321-7339.
  2. 大澤　朗、「腸管モデルの開発で、科学的実験の土台をつくる」，Top Researchers　（オンラインジャーナル），株式会社経営共創基盤 (2019）
  3. 佐々木大介, 佐々木健吾, 近藤昭彦, 大澤　朗、総説、in vitro 培養システムによる食物繊維のヒト腸内細菌叢への影響評価、ルミナコイド研究　 Vol.22 No.2, 63-73(2019)
  4. 大澤　朗、総説、食品成分の機能性・安全性評価の新展開、ー神戸大学腸管モデルの概要 ー、ソフト・ドリンク技術資料、No.1841号、1-14(2018)
  5. 大澤　朗、消化管からみた健康・栄養、日本栄養・食糧学会監修、南久則・宮本賢一・山田耕路（編）、「第6章　腸内細菌と健康・栄養」、103-126、建帛社 (2018)
  6. Hayashi, D., Shirai, Y. (2018) “Protective Role of Alpha-Tocopherol in Diabetic Nephropathy” In Nutritional and Therapeutic Interventions for Diabetes and Metabolic Syndrome. p.375-383. Ed. Debasis Bagchi, ELSEVIER.