Biologics Research Unit Projects

Large‐Scale Research Projects

  1. AMED Project Focused on Developing Key Technology for Discovering and Manufacturing Drugs for Next-Generation Treatment and Diagnosis Next-generation Biologics Manufacturing Conforming to Global Standards(2018-2023)Uchida, K.
  2. AMED Research on Development of New Drugs Development of Human Resource Development Programs for Quality Control of Biopharmaceuticals(2018-2020)Kondo, A., Ishii, J., Nakamura, Y., Nishimura, Y.
  3. Ministry of Health, Labor and Welfare: FY2020: Subsidy for operating expenses for accelerated examination of pharmaceuticals, etc. (Pharmaceutical Regulation Training Project)Uchida, K.
  4. AMED Project Focused on Developing Key Evaluation Technology: Evaluation for Industrialization in the Field of Regenerative Medicine「International joint research for virus safety using High throughput sequencing in cell processed therapeutic products」(2019-2021)Uchida, K.

Therapeutic Antibody, Gene Therapy and Peptide Pharmaceutical Research Group

  1. Ito, Y., Terai, G., Ishigami, M., Hashiba, N., Nakamura, Y., Bamba, T., Kumokita, R., Hasunuma, T., Asai, K., Ishii, J.*, Kondo, A. * (2020) Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression, Nucleic Acids Research, 48(22), 13000-13012
  2. Nakamura, Y., Asama R., Tabata, T., Morita, K., Maruyama, T., Kondo, A., Ishii, J.* (2020) Comparative analyses of site-directed mutagenesis of human melatonin MTNR1A and MTNR1B receptors using a yeast fluorescent biosensor, Biotechnology and Bioengineering, in press
  3. Hashi, H., Nakamura, Y., Ishii, J.*, Kondo, A. (2018) Modifying expression modes of human neurotensin receptor type 1 alters sensing capabilities for agonists in yeast signaling biosensor, Biotechnology Journal, 13(4), e1700522
  4. Nakamura, Y., Nishi, T., Noguchi, R., Ito, Y., Watanabe, T., Nishiyama, T., Aikawa, S., Hasunuma, T., Ishii, J.*, Okubo, Y., Kondo, A.* (2018) A stable, autonomously replicating plasmid vector containing Pichia pastoris centromeric DNA, Applied and Environmental Microbiology, 84(15), e02882-17
  5. Ito, Y., Watanabe, T., Aikawa, S., Nishi, T., Nishiyama, T., Nakamura, Y., Hasunuma, T., Okubo, Y., Ishii, J.*, Kondo, A.*(2018) Deletion of DNA ligase IV homolog confers higher gene targeting efficiency on homologous recombination in Komagataella phaffii, FEMS Yeast Research, 18(7), foy074
  6. Nambu-Nishida, Y., Nishida, K., Hasunuma, T., Kondo, A. (2018) Genetic and physiological basis for antibody production by Kluyveromyces marxianus. AMB Express, 8: 56

Virus Safety Research Group

  1. Yusa, K., Yuan, Y. Uchida, K. (2020) Viral safety testing for biopharmaceuticals: Current and future prospects. Translational and Regulatory Sciences. in press


  1. Nakamura, Y., Kondo, A., Ishii, J.* Biosensing techniques in yeast: G-protein signaling and protein-protein interaction assays for monitoring ligand stimulation and oligomer formation of heterologous GPCRs, Peripheral Membrane Proteins, ISBN 978-1-78923-513-5, IntechOpen, Chapter 3, 25-48(2018)