Functional Food Material and Agrobio Research Unit Projects

Large‐Scale Research Projects

  1. MEXT Creation of Innnovation Center for Advanced Interdisciplinary Research Areas Program Innovative BioProduction Kobe (iBioK)(2008-2018)Ashida, H., Osawa, R., Kanamaru K., Mizuno, M., Hashimoto, T., Yamasaki, M., Honda, K.

Agrobio Research Group

  1. Komatsu, A., Ohtake, M., Shimatani, Z., Nishida, K. (2020) Production of herbicide-sensitive strain to prevent volunteer rice infestation using a CRISPR-Cas9 cytidine deaminase fusion., Front Plant Sci., 11: 925
  2. Seki, K., Komatsu, K., Tanaka, K., Hiraga, M., Kajiya-Kanegae, H., Matsumura, H., Uno, Y. (2020) A CIN-like TCP transcription factor (LsTCP4) having retrotransposon insertion associates with a shift from Salinas type to Empire type in crisphead lettuce (Lactuca sativa L.). Hort Res., 7:15(14 pages)
  3. Fujikura, U., Ezaki, K., Horiguchi, G., Seo, M., Kanno, Y., Kamiya, Y., Lenhard, M., Tsukaya H. (2020) Suppression of class I compensated cell enlargement by xs2 mutation is mediated by salicylic acid signaling. PLoS Genet., 25;16(6):e1008873.
  4. Furuhata, Y., Sakai, A., Murakami, T., Morikawa, M., Nakamura, C., Yoshizumi, T., Fujikura, U., Nishida, K., Kato, Y. (2019) A method using electroporation for the protein delivery of Cre recombinase into cultured Arabidopsis cells with an intact cell wall. Sci Rep., 15;9(1):2163.
  5. Ohno, R., Teramura, H., Ogino, C., Kondo, A., Takumi, S. (2018) Genotypic effects on sugar and by-products of liquid hydrolysates and on saccharification of acid-insoluble residues from wheat straw. Genes Genet Syst., 93(1):1-7
  6. Shimatani, Z., Fujikura, U., Ishii, H., Matsui, Y., Suzuki, M., Ueke, Y., Taoka, K., Terada, R., Nishida, K., Kondo,A. (2018) Inheritance of co-edited genes by CRISPR-based targeted nucleotide substitutions in rice., Plant Physiol Biochem., 131: 78-83
  7. Shimatani, Z., Fujikura, U., Ishii, H., Terada, R., Nishida, K., Kondo, A. (2018) Herbicide tolerance-assisted multiplex targeted nucleotide substitution in rice, Data in Brief, 20: 1325-1331
  8. Ishibashi, M., Nabe, T., Nitta, Y., Tsuruta, H., Iduhara, M., Uno, Y. (2018) Analysis of major paralogs encoding the Fra a 1 allergen based on their organ-specificity in Fragaria × ananassa. Plant Cell Report, 37(3):411–424
  9. Omidbakhshfard, MA., Fujikura, U., Olas, JJ., Xue, GP., Balazadeh, S., Mueller-Roeber, B. (2018) GROWTH-REGULATING FACTOR 9 negatively regulates arabidopsis leaf growth by controlling ORG3 and restricting cell proliferation in leaf primordia. PLoS Genet 9;14(7):e1007484

Functional Food Material Intestinal Dynamics Research Group

  1. Yoshida, N., Yamashita, T., Kishino, S., Watanabe, H., Sasaki, K., Sasaki, D., Tabata, T., Sugiyama, Y., Kitamura, N., Saito, Y., Emoto, T., Hayashi, T. Takahashi, T., Shinohara, M., Osawa, R., Kondo, A., Yamada, T., Ogawa, J., Hirata, K. (2020) A possible beneficial effect of Bacteroides on faecal lipopolysaccharide activity and cardiovascular diseases. Scientific Reports, 10, Article number: 13009.
  2. Hayashi D., Wang L., Ueda S., Yamanoue M., Ashida H., Shirai Y. (2020) The mechanisms of ameliorating effect of a green tea polyphenol on diabetic nephropathy based on diacylglycerol kinase α. Sci Rep., 10:11790-11802
  3. Mizuno, M., Sakaguchi, K., Sakane, I. (2020) Oral administration of fucoidan can exert anti-allergic activity after allergen sensitization by enhancement of galectin-9 secretion in blood. Biomolecules, 10, 258,
  4. Oka, S., Ohto, N., Kuwahara, H., Mizuno, M. Oral (2020) administration of pineapple glucosylceramide improves defective epidermal barrier function by restoring diminished level of TGF-β in the skin, Eur. Food Res. Technol., 246, 867-874.
  5. Fujii, M., Fukuura, K., Ohto, N., Kuwahara, H., Mizuno, M. (2020) Lactobacillus plantarum 22A-3 exerts anti-allergic activity through TGF-β secretion in passive cutaneous anaphylaxis of mice, Int. J. Food Sci. Nutr.,
  6. Yoshida, N., Sasaki, K., Sasaki, D., Yamashita, T., Fukuda, H., Hayashi, T., Tabata, T., Osawa, R., Hirata, K., Kondo, A. (2019) Impact of resistant starch on the gut microbiota and its metabolites in a single-batch fermentation system varies among fecal samples from patients with coronary artery disease. Journal of Atherosclerosis and Thrombosis, 26 :705-719.
  7. Shinohara, R., Sasaki, K., Inoue, J., Hoshi, N., Fukuda, I., Sasaki, D., Kondo, A., Osawa, R. (2019) Butyryl-CoA:acetate CoA-transferase gene associated with the genus Roseburia is decreased in the gut microbiota of Japanese patients with ulcerative colitis. Bioscience of Microbiota, Food and Health, 38: 159-163.
  8. Minato, K., Laan, L.C., van Die, I., Mizuno, M. (2019) Pleurotus citrinopileatus polysaccharide stimulates anti-inflammatory properties during monocyte-to-macrophage differentiation, Int. J. Biol. Macromol., 122, 705-712.
  9. Sasaki, D., Sasaki, K., Ikuta, N., Yasuda, T., Fukuda, I., Kondo, A., Osawa, R. (2018) Low amounts of dietary fibre increase in vitro production of short-chain fatty acids without changing human colonic microbiota structure. Scientific Reports, 8, Article number: 435
  10. Sakaguchi, K., Shirai, Y., Itoh, T. Mizuno, M. (2018) Lentinan exerts its anti-inflammatory activity by suppressing TNFR1 transfer to the surface of intestinal epithelial cells through Dectin-1 in an in vitro and mice model, Immunome Res., 14, 165. DOI: 10.4172/1745-7580.1000165.
  11. Hayashi, D., Ueda, S., Yamanoue, M., Ashida, H,. Shirai, Y. (2018) Amelioration of diabetic nephropathy by oral administration of d-α-tocopherol and its mechanisms. Biosci Biotechnol Biochem., 82, 65-73.

Functional Food Material Disposition Research Group

  1. Yamashita, Y., Wang, L., Nakamuea, A., Nanba, F., Saito, S., Toda, T., Nakagawa, J., Ashida, H. (2020) Black soybean improves the vascular function through an increase in nitric oxide and a decrease in oxidative stress in healthy women. Arch Biochem Biophys, 688, 1080408.
  2. Yamashita, Y., Nakamuea, A., Nanba, F., Saito, S., Toda, T., Nakagawa, J., Ashida, H. (2020) Black soybean improves vascular function and blood pressure: A Randomized, placebo controlled, crossover trial in humans. Nutrients, 12, 2755.
  3. Wang, L., Yamashita, Y., Komeda, S., Saito, A., Ashida,H. Absorption, metabolism, distribution and faecal excretion of B-type procyanidin oligomers in mice after a single oral administration of black soybean seed coat extract. Food Funct, 9, 5362-5370 (2018)


  1. Yamashita, Y., Sakakibara, H., Toda, T., Ashida, H. (2020) Insights into the potential benefits of black soybean (Glycine max L.) polyphenols in lifestyle diseases. Food Funct, 11, 7321-7339.
  2. Hayashi, D., Shirai, Y. (2018) “Protective Role of Alpha-Tocopherol in Diabetic Nephropathy” In Nutritional and Therapeutic Interventions for Diabetes and Metabolic Syndrome. p.375-383. Ed. Debasis Bagchi, ELSEVIER.